SciPro is retained again with one of our favortire clients again as they grow their R&D site in Boulder, CO. The primary role of this position is to support our small-molecule drug discovery programs by delivering ligand-protein co-crystal structures through gene-to-structure process. This individual will work closely with scientists from multiple disciplines including enzymology, medicinal chemistry, and computational chemistry. Career path of this position initially focuses on as a bench-side, individual contributor with growth potential into a mentoring /managerial role based on career interest, accumulated experience, and proven performance.
Purify recombinant protein suitable for protein crystallography and in vitro assays
Utilize multiple crystallization formats to grow and optimize diffraction-quality ligand-bound protein co-crystals.
Collect and process datasets using both in-house X-ray generators and remoted data collection on synchrotrons with optimized collection strategies.
Solve and refine structures either by Molecular Replacement or de-novo
Disseminate the results to project teams
Assist Structural Biology colleagues with challenging protein targets.
PhD in structural biology or a related field having 5+ years’ postdoctoral experience with crystallography in industrial or academic research that leads a proven track record of achievement and publications in reputable, peer-reviewed journals.
Demonstrated competency in protein expression and purification strategies using bacterial, insect, and/or mammalian hosts.
Extensive experience in protein X-ray crystallography from crystallization, optimization, data collection and processing, to structure solution.
Experience with software for model building and refinement such as Phenix, Autoproc, CCP4, Chimera, Rosetta, Coot.
Experience with crystallization robot and standard analytical techniques associated with assessing protein quality during the purification process.
Provide regular updates to cross-functional teams to support structure-based compound design in close collaborations with medicinal and computational chemists.
Excellent written and oral communication skills.
Experience with Linux OS and GPU-accelerated computational environment.
Experience to use integrated biophysical and structural biology experiments to understand interactions between small molecules and macromolecular target.
Experience with cryo-EM sample preparation protocols and data collection/processing.